Maydavian Online Reservation and Booking System Essay Example for Free

Maydavian Online Reservation and Booking System Essay Maydavian has been founded as a resort in the beautiful island of Palawan. With the exertion of Ms. Marrieta S. Mayor, the capital of this unspoiled island, and a memorable journey of about two hours by bus or shuttle taking you through a zigzag in the mountains and with breathtaking views of mountain ranges and unspoiled forests. An oasis of hospitality awaits you at Maydavian, with spectacular views of the Sulu Sea. Ideally located in a coconut plantation with modern facilities in harmony with the serene and soothing atmosphere. Maydavian Resort establish on 2000. The establishment started with just a small canteen, 6 rooms and small dinning cottages. After a few months, reception hall, VIP rooms and Standards rooms were built. A swimming pool was formally open to the public. Maydavian Resort has a total land area of eighteen hectares located at Brgy. Caguisan Narra, Palawan. The guest who visited Maydavian loved enjoyed a walkway through century mangroves, clear Sky and myriad of stars and delicious food serves for the most discerning of pallets comprising both Filipino and western specialties. Presently, Maydavian offers plenty of facilities and services. There are the Restaurant and Bar, the Maydavian Pavillion. One of the venues for wedding and birthday parties, island Park intended for playful kids and weather favors the park also serve as venue for children party and garden wedding reception for sports minded guest, there is a room venue for billiards and many more that had contributed to the overall success of this more than a decade old establishment. The continuous growth of the world their exist the modern and a high-tech way of living due to the reason of unsatisfied and consciousness of humans by discovering and searching for more effective way of living such as computer- is a device that computes, especially a programmable electronic machine that performs high speed mathematical or logical operations that can assembles, store, correlates and otherwise process information. Dealing with the triumphant advancement, internet is a network of almost transaction system through this transaction system must gather sort of information for online services. Website is related collection of web files that includes an initial called a homepage it is essential in an organization to establish a tourist spot in the province of Palawan adopting reliable reviewing like a website to provide a complex transaction system through on line services. This system is entitled â€Å"Maydavian Resort Online Reservation and Booking System†.

Wagner Matinee Essay Example for Free

Wagner Matinee Essay During the 1800s there was an increase in immigration to the Great Plains due to an abundance of free land offered by the Homestead Act. Many individuals and families went west to seek their fortune and a home on what was once called the great American desert. Moving westward was a difficult process, and many were forced to leave their old lives behind completely. In A Wagner Matinee, written by Willa Cather, the characterization of Aunt Georgiana, a woman who leaves Boston to elope with a young man going west, is used to illustrate the theme; that sometimes decisions need to be made, and although one might regret it, one love must be sacrificed for another. One way that Cather uses characterization to illustrate this theme is through direct statements about Georgiana and physical descriptions. Right away Cather tells us that Georgiana is, or was, a pianist of some accomplishment. She once worked as an instructor at the Boston Conservatory of Music. For a woman to achieve this status in the time A Wagner Matinee is set was an extraordinary thing. The music she chose to give up was surely something she loved very dearly and was very proud of. The physical description of Aunt Georgiana shows the hardships she had to endure because of her sacrifice. The narrator of the story, Georgianas nephew Clark, describes her as having yellow and leathery skin, and wearing ill-fitting false teeth. These physical defects were the result of a pitiless wind and the alkaline water, along with other hardships of the Nebraska frontier. The most biting description of Aunt Georgiana is the repeated image of her hands. She was once a great pianist, but from work on the farm in Nebraska, her hands have been reduced to twisted knots of flesh, with oddly bent, tentacle-like fingers. This description best illustrates her sacrifice, as her hands were the most vital thing to her musical essence, and Cather does well to convey this through the repeated imagery. Georgianas statements throughout the novel also support the theme of regret and sacrifice. Clark recalls a time when Georgiana opens her soul to him, and says Dont love it so well Clark, or it may be taken from you. Oh! Dear boy, pray that whatever  your sacrifice be it is not that. By saying this she is referring to, of course music. Her advice to not love it so well is intensely pessimistic, influenced by the great pain she suffere d when she gave up her life ambition. Later in the only conversation that Clark and Georgiana share at the concert, she remorsefully makes reference to the music they are enjoying. And youve been hearing this ever since you left me, Clark? she asks. Clark calls the question the gentlest and saddest of reproaches. When she says this she is almost jealous of her nephew. Her wistful remembrance and longing is made clear through her words. Finally, at the end of the concert, she bursts into tears and cries out I dont want to go Clark, I dont want to go!† This is a very direct and clear example that her longing for the music she sacrificed is terrible and immense. However, Aunt Georgianas actions often speak volumes, and impart a greater insight into the theme than even her words can convey. She had lived on a farm away from civilization for thirty years, and so the transition back into the city was not an easy one. She is described as being almost in a trance when she arrives. Her state of being does not change throughout the story until she arrives at the matinee and she awakens. The only thing that connects her back to the city she left so long ago is the m usic. When the concert starts, it opens the flood gates to her memories. The very moment the first note is played her hand darts out and clutches at Clarks coat sleeve. She does not release it until that song is has come to an end. After this first ordeal she is able to calm herself somewhat, but the music still controls her, seeming to sweep her away to another world. As the concert continues, she fondly remembers her days at the conservatory as she pantomimes the action of playing a piano on her dress. Through these actions and others she demonstrates how dear to her the music that she left behind is. Thus, Cather uses the characterization to illustrate the theme of sacrifice. The vivid physical description presented makes the weight of Georgianas decision clear. Georgianas words also provide great insight into how she feels. Indirect expressions, which can be interpreted through her conscious and subconscious actions, are the most solid representation of how the theme is demonstrated through characterization. Aunt Georgiana is not a unique case, and although she is a fictional character, sacrifices like hers are inevitably made every day. Sometim es there is no middle ground, and no  matter how great a love is, it must be abandoned for what is believed to be a greater good. The regret and pain which accompany these sacrifices must be dealt with by each individual.

Using Thin Layer Chromatography To Monitor Reactions Environmental Sciences Essay

Using Thin Layer Chromatography To Monitor Reactions Environmental Sciences Essay Thin layer chromatography (TLC) is a very useful technique for monitoring reactions. It can also be used to determine the proper solvent system for performing separations using column chromatography. TLC stationary phases are usually alumina or silica. They are polar for standard experiments or non-polar for reverse phase chromatography. The mobile phase is a solvent whose polarity is chosen by the person conducting the experiment. In most laboratory work standard phase silica plates are used. Different compounds will travel different distances up the plate depending on the polarity of the components of the mixture. The more polar compounds will be more attracted to the polar silica gel and travel shorter distances on the plate. Mon-polar substances will spend more time in the mobile phase and as a result will travel larger distances on the plate. The measure of the distance a compound travels is called the retention factor (Rf ) value. The retention factor, or Rf, is defined as the distance traveled by the compound divided by the distance traveled by the solvent. For example, if a compound travels 2.1 cm and the solvent front travels 2.8 cm, the Rf is 0.75: The Rf for a compound is a constant from one experiment to the next only if the chromatography conditions below are also constant: solvent system adsorbent thickness of the adsorbent amount of material spotted temperature Since these factors are difficult to keep constant from experiment to experiment, relative Rf values are generally considered. Relative Rf means that the values are reported relative to a standard, or it means that you compare the Rf values of compounds run on the same plate at the same time. 1.1 Thin Layer Chromatography There have been a numbered of important milestones in the evolution of chromatography in the last 100 years. Each of these milestones has signalled the start of an important branch of chromatography. Some examples of these are; partition chromatography (1941), gas chromatography (1951-1952), high performance liquid chromatography (mid- 1960s), capillary electrophoresis (1980) and capillary electrochromatography (past decade). In all the chromatographic techniques mentioned, separation is carried out in a column. However, it is also possible to carry out separations on a planar surface. Two examples of this are paper chromatography (1944) and thin-layer chromatography (1937-1938). Thin-layer chromatography (TLC) replaced paper chromatography as the most popular, routine chromatographic technique. TLC was first used in 1937 to 1938 by Nikolai A. Izmailov and Maria S. Shraiber at the Institute of Experimental Pharmacy of the State University of Kharkov. At the time Izmailov was the head of the institute and Shraiber was his graduate student. They were searching for a method for the rapid analysis of galenic pharmaceutical preparations (plant extracts). As classical column chromatography would have taken too much time they felt that if the absorbent would be prepared in the form of a thin-layer on a glass plate. They believed that it would behave like a column but the characterization time would be much shorter. They coated microscope slides with a suspension of various adsorbents (calcium, magnesium and aluminium oxides). They deposited one drop of the sample solution on this layer and added one drop of the same solvent used in a column to develop separation. The test was a success as the separated sample components appeared as concentric rings that fluoresced in various colour s under a UV lamp. They showed that the sequence of the concentric multicoloured rings on the plate would have been identical to the sequence of coloured rings obtained on a normal chromatographic column. They called this technique spot chromatography and the result on the microscope slides ultrachromatograms. The paper on this experiment was published in a Russian pharmaceutical journal that was practically unknown outside the then Soviet Union. Its abstract was included in a Russian review journal and through it in chemical Abstracts. It was then read by M. OL. Crowe of the New York State Department of Health. He then adapted the technique for his own use. Crowe prepared the adsorbent layer in a Petri dish, added a drop of the sample solution in the centre and then added the developing solvent dropwise until sufficient separation was obtained. In 1947 T.I. Williams described a further improvement of the method of Izmailov and Shraiber in his textbook on chromatography. He prepared the adsorbent-coated glass plates in the form of a sandwich. The adsorbent layer was covered by 2 glass plates and had a small hole which the sample drops could be applied through. Meinhard and Hall made the next major step in the development of TLC at the University of Wisconsin. They used corn starch, which acted as a binder, to hold the coating on the glass plate and added a small amount of Celite powder to the adsorbent particles to improve the consistency of the layer. They called this surface chromatography. They used it to separate inorganic ions. Modern TLC started 50 years ago with the work Department of Agriculture Fruit and Vegetable laboratory in Southern California. He investigated the flavour components of the juices of citrus fruits. However, he stated that very large volumes had to be processed because the amount of flavour material was extremely small. Another problem was in finding an analytical method for the investigation of the juice concentrate composition. He followed the method of Meinhard and Hall that he read in Chemical Abstracts. However, instead of adding just a drop of the developing solvent he developed the plates as in paper chromatography. The plates were developed in a closed chamber and one side was dipped into the solvent. The solvent then ascended through the plate by capillary action. It carried with it the sample components and they were separated as a result. The experiments carried out were published and are considered the start of modern TLC. Egon Stahl was responsible for TLC becoming a universally accepted technique. He was also the first to use the term thin-layer chromatography to characterize the technique. This choice of name was almost immediately accepted. Stahl investigated various essential oils and obtained good results using adsorbent-coated glass plates. However, neither the method nor the adsorbent to be used had been optimized. Also, the adsorbents had to be modified and treated before they could be used for the coating of plates. Stahl started investigating the operational parameters and the adsorbent preparations. In the spring of 1958 his efforts were fulfilled as the necessary basic instrumentation, made by Desaga and silica gel G according to Stahl for TLC, made by E. Merck were both introduced at the international Achema exhibition of chemical equipment in Frankfurt. Stahl also published an article outlining the use of the system and a wide range of applications. Because of this standardized method TLC became a widely used laboratory technique. He also went on to publish a TLC handbook in 1962. Although TLC had a wide application it was still thought to be a qualitative technique for the analysis of simple mixtures. As a result advances were directed toward improving the technique. Instrumentation which permitted more precise spotting of the sample onto the plates and the quantitative evaluation of the separated spots was developed. Faster analysis and higher separation power was also achieved. As a result of the higher performance ability the name was change to high-performance TLC (HPTLC) by R.E. Kaiser, who was instrumental in its development. The particle size and range of the adsorbent was the main difference between TLC and HPTLC. The silica gel for TLC had broad particle sizes of 10-60 µm with an average of 20 µm whereas HPTLC has an average of only 5 µm. the HPTLC plates were also smaller in comparison with TLC plates, 10 x 10cm and 20 x 20cm respectively. The improved method and design allowed reduction in the diameter of the starting spots. These improvements lowered the analysis time and increased the efficiency. Problems arose with flow rate which Kaiser overcame by applying pressure to the TLC plate. This in turn led to forced-flow TLC. Due to the constant condensation-evaporation process associated with developing TLC plates in developing chambers problems can be encountered because of the changing velocity of the mobile phase. To overcome this forced-flow TLC (FFTLC) was developed by Tyihà ¡k, Mincsovics and Kalà ¡sz. In this method the spotted plates (dry) are placed into a pressurized development chamber. The stationary-phase layer is tightly covered and sealed on its side by an elastic membrane and pressurized by an inert gas or water filling up the cushion above the layer. The mobile phase is delivered via a pump at a constant velocity through a slit in the membrane to the stationary phase. There are various configurations which can be handled using this method. TLC is a very simple technique. As a result very little instrumentation is needed. Application of samples to the stationary-phase is carried out using a micropipette or syringe. The developing chambers are simple glass structures. Detection is carried out by visual inspection or made visible by spraying the plate with reagent. Also, a wide variety of precoated plates are available so coating equipment isnt needed. In more advanced systems the samples may be spotted by automated loading devices (dosimeters). This allows the application of small and uniform sample spots. More sophisticated developing chambers are also available (FFTLC). The plates can be scanned by densitometers and quantitatively analysed using absorbance or fluorescence measurements. Chromatograms with peaks of the individual separated spots recorded against the length of the plate are produced with such analyses. Their area is also a proportion to the amount present. More complex systems can also be created by combining TLC systems with other systems such as mass spectrometry and Fourier transform infrared. 1.2 Ink Analysis Ink analysis is a very important forensic procedure. It can reveal useful information about questioned document. Modern inks contain many substances which are aimed at improving the ink. The most important component of the ink is the colouring material. It comes in the form of a dye, pigment or a combination of both. Dyes are soluble in the liquid body of the ink, also known as the vehicle. Pigments are finely ground multi-molecular granules that are insoluble in the vehicle. The vehicles composition affects the flowing and drying characteristics of the ink and can consist of oils, solvents and resins. 1.3 Chromatography Studies Djozan et al developed a new and fast method for the differentiation of inks on a questioned document. They designed specific image analysis software for evaluating thin layer chromatograms. They sampled forty-one blue ballpoint pens which were purchased from their local markets in Iran (Table 1). They first wrote a circle of diameter 5 mm uniformly by pen on a paper. One fourth of this was then punched out for extraction. They carried out extraction in 1 ml glass tubes and added 0.1 ml of methanol. This was then vigorously shaken for 1 min. the ink component was then fully dissolved in methanol. The supernatant methanoic solutions were then used to spot the TLC plates. A blank sample of paper with equal dimensions was also treated in the same way. Table 1. List of blue ballpoint pens studied List of blue ballpoint pens studied 1 Cello pyramid 0.7 mm fine TC ball 2 OBA 3 AIHAO 4 Bic 01 5 Cenator 6 PARKER 7 A.T.CROSS FINE 8 Pelikan STICK 918 9 Marvy SB-10 1.0 mm 10 Bic 02 11 PIANO crystal 12 My pen 2001 PENS High Quality Bluce CE 13 AIBA 14 STAEDTLER Stick 430M A IRAN 15 Reynolds Medium 048 France 16 EIFEL Elegance 17 CASPIAN STICK 2001 M 18 STABILO liner 308 19 FABER-CASTELL 1.0 mm Medium (transparent) 20 BIC 08 21 Bocheng A-100 22 SCHNEIDER TOPS 505 M Germany 23 FIBER-CASTELL 1.0 mm Medium 24 MILAN PI 1 mm 25 Reform 26 PAPER = MATE FLEXGRIP ultra MED 27 PARKER UK 28 CANDID-DINI 2853 29 STABILO-galaxy 818 M 30 No name 31 No name 32 Zebra Rubber 101 33 SANFORD SAGA 34 Bensia 35 Girls 36 EUROPEN 37 PARS swiss Refill 606 38 STAEDTLER stick 430 M TBRITAIN 39 Lus HF 500 40 No name 41 STABILO bill 508 TLC analysis was carried out on Merck (Darmstadt, Germany) 20 cm x 20 cm silica gel 60 TLC plates without fluorescent indicator. The plates were activated at 60  °C for 20 min and immediately spotted after cooling in a desiccator. The plates were developed in a developing chamber. The mobile phase used was: ethyl acetate/ absolute ethanol/ distilled water (70:35:30, v/v/v). Chromatographic development of the plates was carried out at room temperature for 40 min. All mobile phases were prepared daily with analytical grade chemicals. Enough was prepared to supply the tank for each run. The plates were air-dried after development. The separated compounds were visible on the plate by their natural colour and the plates were scanned into a computer using an office scanner. An IBM compatible PC (Pentium IV) with a 2.6 GHz microprocessor, 256 MB random access memory (RAM) and a hard disk with 40 GB capacity for external storage was used for processing the colour images. The computer was equipped with an on-board graphic card (NviDiA Geforce 7300LE) and a scanner (CanoScan 4200F) was connected to the computer for scanning (300 dpi) TLC plates as digital images. The images were saved as bmp files. Matlab (Version 6.5, The Math Works, Inc.) was used to write a new program to process the previously saved images. Previous studies indicated that Pyridine is the solvent used with ballpoint pen inks. Djozan et al preformed extraction with different solvents using various extraction modes. These modes were immersion of paper into solvent and simple agitation for 1 min, ultra-sound assisted extraction and micro-wave assistance extraction. The results showed that the immersion of paper into methanol or pyridine and simple agitation resulted in complete extraction of the inks from paper (Table 2). Table 2. List of solvents used for extraction of ink components from paper Solvent Solubility of ink colours Ethyl acetate Ethanol Acetic acid Acetone Butanol 1,2-Dichloroethane Butyl acetate Tetrachloroethane Acetyl acetate Cyclohexane Methanol Pyridine Slightly Slightly Slightly Slightly Slightly Slightly Slightly Slightly Slightly Slightly Soluble Soluble No improvement was found using ultra-sound or micro-wave assisted extraction. Methanol was chosen as the extraction solvent due to the safety of the solvent. The selection of the plate was down to the fact that silica gel plates provided the best resolution of dye spots. They selected five mobile phases (Table 3) and found that ethyl acetate/ absolute ethanol/ distilled water (70:35:30, v/v/v) was effective in separating nearly all the dye mixtures. The spot capacity obtained was more than 15. Table 3. Different solvent systems used to develop plate Solvent System Ratio Spot capacity Butanol:ethanol:H2O Ethyl acetate:cyclohexane:methanol:NH3 Ethyl acetate:Butanol:NH3 Ethyl acetate:ethanol:H2O Toluene:acetone:ethanol:NH3 50:15:10 70:15:10:5 60:35:5 70:35:30 30:60:7:2 9 5 10 15 5 Fig. 1. Typical TLC results of 10 different ink samples (Djozan et al, 2008) Fig. 1. shows a typical chromatogram that they achieved in their experiment. To confirm complete separation of all components in the studied sample, two-dimensional (2D) TLC was carried out using various solvent systems. The results proved that the one-dimensional (1D) TLC is able to provide sufficient separation. The first stage carried out was colour image normalization. A function of the input images was computed that is invariant to confounding scene properties but was discriminative with respect to desired scene information. The calculation is as follows: Stage 2 is to compute a colour image profile. The intensity profile of an image is the set intensity values taken from regularly spaced points along a line segment in an image the intensity values are interpolated for points that dont fall on the centre pixel they computed an intensity profile for r, g and b images along the line passing through the centre of the image on the chromatographic development straight of each ink spot. Fig. 2. RGB characteristic of an ink after TLC (Djozan et al, 2008) In stage 3 the colour image profiles were correlated. The intensity profiles were considered as sequences and the normalized cross-correlation of sequences were computed. Cross-correlation is a measure of similarity of two signals. It is used to find features in an unknown signal and compared to a known signal. It is calculated as follows for discrete functions: Eq. (1) For image-processing applications in which the brightness of the images can be due to lighting and exposure conditions, the images can be first normalized. It is calculated as follows: Eq. (2) Stage 4 involved computing image similarity. The weighted mean of and were computed as follows: Eq. (3) The ability of the method to differentiate between various blue ballpoint pens was evaluated by comparing the similarity of different inks according to Eq. (3). Fig. 3. Screen shot of Matlab software running (Djozan et al, 2008) Fig. 4. All possible combination of comparing inks with TLC-IA (Djozan et al, 2008) In 2006 Liu et al published a paper on the classification of black gel pen inks by ion-pairing high-performance liquid chromatography. They stated that black gel inks usually contain several dye components. These components all have different colours and are mixed together proportionally to give the black colour. They used reverse-phase ion-pairing high performance chromatography (RP-IP-HPLC). It was done in such a manner as the dyes couldnt be reversed on the C18 column due to their high polarities. The maximum UV absorption bands of the black gel pen inks obtained were between 500 and 700 nm. The wavelength of the detector was set to 580 nm as most of the dyes had a maximum UV adsorption near 580 nm. They investigated the influence of both volatile and non-volatile ion-pairing reagents on the HPLC analysis of black gel pen ink dyes. All the reagents had different alkyl chain, ammonium acetate, triethylamine (TEA), tributylamine (TBA), dihexylamine (DHA) and tetrabutylammonium bromide (TBABr). The results revealed that the dyes were nearly not retained using ammonium acetate or TEA as the ion-pairing reagent. Using TBABr, TBA and DHA as the ion-pairing reagent, individually, the dyes were separated. TBABr was selected as the ion-pairing reagent as the retention times were shorter than the others and sharper peaks were obtained. They also investigated the buffer solution concentrations and the effect of pH on the separation. The optimum result was: 40 mmol/L TBABr buffer solution (pH 7) with acetonitrile as the organic modifier for IP-HPLC analysis and an identical proportion of the buffer and acetonitrile (v/v = 40:60) at a flow rate of 1.0 mL/min. these optimum conditions were used to separate 50 dye-based black gel pen inks by IP-HPLC. Liu et al carried out another study on ion-pairing HPLC in 2006. This time, however, they studied the degradation of blue gel pen dyes and also used electrospray tandem mass spectrometry. They used ion-pairing reversed phase liquid chromatography as the inorganic compounds they were analysing have weak retention on the ordinary reversed stationary phases when separating on HPLC. This is due to their high polarities. The UV detector was set at 580 nm for the analysis as most dyes have a normal maximum absorption near 580 nm. The UV absorptions of the fluorescence whitening reagents in paper are usually below 500 nm and they had no interference for the detection of the gel pen dyes at 580 nm. Fig. 5. Chromatograms of blue gel pen inks using different ion pairing reagents (Liu et al, 2006) The tested various mobile phases: eluent A: eluent B (acetonitrile) = 50:50 (v/v); eluent A was the buffer of ion pairing reagent with concentration of 40 mmol Là ¢Ã‹â€ Ã¢â‚¬â„¢1 (pH 7.0), and the ion pairing reagent was (a) ammonium acetate, (b) TEA acetate, (d) TBA acetate, (e) DHA acetate and (f) TBABr, respectively. (c) Ammonium carbonate as eluent A (40 mmol Là ¢Ã‹â€ Ã¢â‚¬â„¢1, pH 9.5) and eluent A:eluent B (acetonitrile) = 50:50 (v/v). they found that 10 mmol-1 TBA acetate (pH 7.0) was suitable ion-pairing agent for the purpose and ink samples stored in different conditions were analyzed by IP-HPLC. Significant changes of ink composition were observed. The noticed that the natural aged inks had the similar but weaker degradation trend than the light aged inks. They used HPLC-MS/MS with ammonium carbonate as ion-pairing reagent to obtain the information of the light aged inks and their photodegradation mechanism. In 1994, Varshney et al analysed ink from typed script of electronic typewriters by HPTLC. They used script from seven electronic typewriters. They used the resultant Rf values and in-situ visible spectra of each resolved band of all the chromatograms indicated that the same chemical composition is being used in six typewriter ribbon inks. However, the seventh one is completely different. Fig. 6. Wavelength maxima values of in-situ visible spectrum bands of electronic typewriter scripts (Varshney et al, 1994) Fig. 6. shows the densitograms obtained after scanning and integration of the chromatograms of tracks of individual typewriters and blank paper. The seven electronic typewriter inks could be categorised into two groups after analysis. The first group resolved the sample to four bands including the base. The second group did not resolve the samples at all with the solvent systems used. Several varieties of blue ballpoint pen inks were analysed by HPLC and IR spectroscopy by Kher et al in 2006. The chromatographic data extracted at four wavelengths (254, 279, 370 and 400 nm) was analyzed individually and at a combination of these wavelengths by the soft independent modeling of class analogies (SIMCA) technique. They used principal components analysis (PCA) to estimate the separation between the pen samples. Linear discriminant analysis (LDA) measured the probability with which an observation could be assigned to a pen class. The best resolution was obtained by HPLC using data from all four wavelengths together, differentiating 96.4% pen pairs successfully using PCA and 97.9% pen samples by LDA. PCA separated 60.7% of the pen pairs and LDA provided a correct classification of 62.5% of the pens analyzed by IR. They stated that HPLC coupled with chemometrics provided a better discrimination of ballpoint pen inks compared to IR. Kher et al effectively combined LDA and PCA to classify the HPLC and IR data. PCA gives a general idea of how different a given pair of pens is, whereas LDA can quantify the predictive ability of a generated classification model. The two techniques of PCA and LDA were shown to be complimentary to each other. The PCA and LDA results indicated that although IR cannot differentiate between all classes of pen inks, it can still provide a reasonable discrimination, which can be enhanced further by improving the quality of the spectra. The analysis of such an enhanced IR data with chemometric analyses would provide a valuable non-destructive tool for forensic analyses. Raman Spectroscopy Studies Mazzella and Buzini used Raman spectroscopy to analyse blue gel pen inks in 2004. They sampled 55 blue gel pens. They first separated them into two groups using a preliminary solubility test in methanol. They discovered that 36 were pigmented inks, which arent soluble in methanol, and 19 were dye-based inks, which are soluble in methanol. They applied Raman spectroscopy to the 36 pigmented blue gel inks. Raman spectroscopy is a non-destructive technique. Spectra were first obtained using the 514.5 nm argon ion laser which proved the observation of 4 different groups. They then used the 830 nm NIR diode laser and divided the inks into three groups. They then combined the two lasers and a separation into 5 groups was obtained. They then attempted to identify the pigments contained in the gel by comparison to standard pigments. Two main pigments were detected in the analysed samples: PB15 and PV23. PB 15 is pigment blue 15 and belongs to the class of phthalocyanines. PV23 is the pigment violet 23 and belongs to the class oxazines. The argon laser allowed the detection of a mixture of PB 15 and PV 23. This was a better result than using the NIR diode laser. The results showed that the same gel pen ink (same model and brand) from different geographical locations showed the same Raman spectra. However, it was stated that the Raman technique obtained low discriminating values. 2. Materials and Method 2.1 Materials: Blue ballpoint pens Merck silica gel 60 TLC plates (20 cm x 20 cm) Methanol Ethyl acetate Ethanol (absolute) Paper Dessicator Developing chamber Puncher Glass tubes (0.1 ml) Capillary tubes 2.2 Experimental 13 blue ball-point pens (Table 1) were bought from a number of different shops in the town. A circle with a diameter of 5mm was written by the pen on paper. One fourth of it was punched out for extraction. The samples are placed in 1 ml glass tubes. 0.1ml of methanol was added and vigorously shaken for 1 min. The ink component was fully dissolved in methanol. The supernatant methanoic solutions were used for spot application on TLC plate. A blank of paper only is also treated as was a control which was a permanent marker. TLC analyses were preformed using Alugram 20 cm x 10 cm silica gel/UV plates (Macherey-Nagel). The plates were activated at 60 °C for 20 min and immediately after, cooled in a desiccator, and spotted. The plates were developed in a horizontal developing chamber. The solvent system included: ethyl acetate/absolute ethanol/ distilled water (70:35:30, v/v/v). Development was preformed at room temperature for 40 min. All mobile phases were prepared daily. After develo pment the plates were air-dried. All 13 different pens were tested in triplicate. Retention factors were calculated using the results from the plates and photographs taken using a digital camera were loaded onto the computer and analysed using image analysis software. Table 1: List of pens analysed Number Description 1 No Brand (blue) 2 Pilot G-207 3 BIC ReAction 4 BIC Medium (Bought in Tesco) 5 BIC Medium (Bought in Dunnes) 6 No Brand (Purple) 7 Staedtler Stick 430M 8 Roller Pen 9 Papermate 1.2M 10 Scripto Stick Pen 11 Papermate Write Bros. 12 Comfort Touch 13 No Brand (Tesco Click Pen) 3. Results and Discussion Before carrying out the experiment it needed to be researched. This research pointed out the importance of the correct solvent to remove the ink from the document. Djozan et al used methanol as their choice of solvent after considering other solvents (Table 2). They stated that Pyridine was the reported solvent used with ball-point pen inks. However, they carried out extractions with different solvents using various extraction modes. They realised that immersion of the paper into methanol with agitation resulted in complete extraction of the inks from the paper. Methanol was also chosen because of its safety. Table 2: List of solvents used for the extraction of ink components from paper Solvent Solubility of ink colours Ethyl acetate Slightly Ethanol Slightly Acetic Acid Slightly Acetone Slightly Butanol Slightly 1,2-Dichloroethane Slightly Butyl acetate Slightly Tetrachloroethane Slightly Acethyl acetate Slightly Cyclohexan Slightly Methanol Soluble Oyridine Soluble Different concentrations of the solvent system (Table 3) were analysed to see which gave the greater separation. It was found that the concentration given by Djozan et al, (ethyl acetate, ethanol, and water (70:35:30, v/v/v)) gave the best results. The Alugram silica Gell/UV plates were also found to work better than the suggested, Merck silica gel 60, plates without fluorescent indicator. Table 3: Concentrations of solvent system investigated Number Solvent system 1 ethyl acetate, ethanol, water (70:35:30, v/v/v) 2 ethyl acetate, ethanol, water (70:30:35, v/v/v) 3 ethyl acetate, ethanol, water (70:25:40, v/v/v) 4 ethyl acetate, ethanol, water (70:40:25, v/v/v) Table 4: Retention factors for all separated components pen spot 1 spot 2 spot 3 spot 4 spot 5 Solvent RF1 RF2 RF3 RF4 RF5 1.1 70 73 0.958904 1.2 70 73 0.958904 1.2 70 73 0.958904 2.1 62 69 73 0.849315 81.24194 2.2 62 69 73 0.849315 81.24194 2.3 62 69 73 0.849315 81.24194 3.1 57 60 62 70 0.814286

Damsels In Address Essay -- essays research papers fc

Damsels in Address It is clearly evident that many fairy tales of childhood tend to shape the reader. Certain moral codes and ideals are tightly woven into the text of many fairy tales, promoting or denoting a character’s actions. In the Grimm’s fairy tales Cinderella, Brier Rose, and Rapunzel, the heroines of these tales exhibit strong behavioral codes, thus providing opportunity for the young female reader to relate to the damsel, or to model herself to behave in a similar fashion. In accordance with Marcia R. Lieberman’s essay, " ‘Some Day My Prince Will Come’: Female Acculturation Through the Fairy Tale," I agree with the assertion that positive traits in fairy tale indicate reward, while the negative characteristics bring misfortune. A heroine in a fairy tale is to be seen as a mentor, a model to easily portray what is right, and what is inherently wrong. For instance, a passive heroine proves to bring eventual reward through pain and suffering, while a female who is assertive, either mentally or physically, is shunned. Suggestions integrated throughout the text of the three tales provide strong evidence as to the desired morals and values of the society in which the tales were written. Through the examination of tales, their inherent messages surface. Children’s perceptions of fairytales can go a long way towards shaping social interactions among said children. Passivity is a major player in the personalities of Rapunzel, Cinderella, and Sleeping Beauty. Rapunzel relies completely on a determined prince to escape her imprisonment; Cinderella uses a fairy godmother to help her cause and Sleeping Beauty waits until Prince Charming wakes her. Children could see these characterizations of women and begin to intertwine them with their own budding personalities. Boys begin to see women as weak and Girls may interpret these behavior traits as indicative of their being the lesser part of relationships with men. S exual roles, although not overtly discussed within the pages of fairytales, becomes the focus for these young people. Marcia Lieberman reiterates the idea of inherent roles stating, "a picture of sexual roles, behavior psychology, and a way of predicting outcome or fate according to sex"(Lieberman, 384). As they grow older, the children may begin to fall into the roles they discovered in the fairytales; boys begin ... ...uch beauty, the maiden is chosen, married, and loved by the hero. This process leads not only to beauty by means of passivity, but also to security and happiness. Assertion equates not only with beauty, but ugliness and misfortune as well. Boys in turn develop a sense of responsibility and see themselves in the dominant role. In other words, they must save the day by getting the girl and then of course spreading wealth. For the young girls of the fairy tale audience to acknowledge that they must act out roles similar to the behavior of the heroines in the three tales under examination, thus guaranteeing eternal wealth and happiness. Boys will see the same roles played out and revert to the breadwinning hero role. Lessons portrayed throughout the story are epitomized by Marcia Lieberman to support the notion of love, success, and failure. Bibliography Works Cited Grimm, Jacob and Wilhelm Grimm. The Complete Fairy Tales of the Brothers Grimm. Trans. and with an introduction by Jack Zipes. 2 vols. Toronto: Bantam Books, 1988. Lieberman, Marcia R. " ‘Some Day My Prince Will Come’: Female Acculturation Through the Fairy Tale." College English 34 (1972/73): 383-395.

Considering The Strange Case of Dr. Jekyll and Mr. Hyde as an Effective

Considering The Strange Case of Dr. Jekyll and Mr. Hyde as an Effective Representation of Evil The Strange Case of Dr. Jekyll and Mr. Hyde, originally published in 1886 by Robert Louis Stevenson, arguably remains a popular novella even today because of its representations of evil and themes concerned with evil such as morality. Originally written for a Victorian audience, the text follows the conventions of the time - for example, the Georgian style of introducing and portraying characters by their social class and status. In writing his classic, Stevenson wanted to "focus on the suggestion that evil is potentially more powerful than good"1 - an idea which would have been out of place then. Indeed, though the Victorian era was a period of great scientific advancement, society was still firmly routed in religion: Sunday would be reserved for Church, a copy of the Bible would be the only book possessed by many, and blasphemy was considered both morally and legally a major crime. Though Charles Darwin had challenged the Biblical theory of Creation some seven years earlier in his thesis The Origin of Species, his works were generally not accepted by the majority of the population - it is extremely likely, as well as logical, that most of his support would have come from the scientific community, which is ironic considering that Dr. Jekyll and Mr. Hyde focuses around the medical profession. Therefore, by focusing on the suggestion that "evil is potentially more powerful than good" 1 - i.e. that God was not almighty or prevalent - Stevenson risked not only the popularity of his novella, but of his reputation as an author. The defi... .../ 3 See: Stafford News 4 Mike Wolfe, Lord Mayor of Stoke-on-Trent 5 Wikipedia [www] http://www.wikipedia.org/ 6 Genesis 4:11 - The Bible (The King James Version) 7 The Inland Voyage, Robert Louis Stevenson 8 Classic Notes [www] http://www.gradesaver.com/ClassicNotes/Titles/jekyll/ 9 Essay Crawler [www] http://www.essaycrawler.com/viewpaper/55322.html/ 10 Dr. Jekyll and Mr. Hyde Criticism [www] http://www.enotes.com/dr-jekyll/7417/ 11 The Carew Murder Case 12 Wikiquote [www] http://www.wikiquote.com/ 13 Peter Bell the Third, P.B. Shelly 14 Wikipedia [www] http://www.wikipedia.org/ and Dr. Mumtaz Pardhan 15 The Placebo Effect: Harnessing Your Mind's Power to Heal [www] http://www.sciencedaily.com/releases/2003/12/031231084101.htm. 16 Leil Lowndess, Relationship Psychologist

The Future of Cyborgs Essay -- Technology Robots

The Future of Cyborgs Terminator and Bladerunner, portrayed cyborgs or cybernetic organisms as creatures of destruction. Are they really as horrible as the movies make them out to be? They can be more useful than perceived; it is necessary to first perfect the technology involved in creating and operating them. In this paper, I will describe how these cyborgs work and how they are portrayed in the movies. Furthermore, I will explain the helpful ways that they are expected to perform in the future. Cyborgs are a very complex creation of the future. The general concept is that they cannot be recognized as non-humans. Although it has a programmed mission, this unit thinks and reacts on its own. The understructure is made of a very strong material that resists many dangers; for example, gunshots and fire. Cyborgs are a self-contained unit under a layer of human flesh. The layer of human flesh that covers the frame is a biological organism. It has different layers and has a capillary system that is flowing with blood. Basically, a cyborg is undetectable to a human without special means and equipment. Cyborgs are portrayed as an evil force in Terminator and Bladerunner. In Terminator, the cyborg is a killing machine sent back in time from the future. The mission of the cyborg is to terminate the mother of a rebellion leader before he is born. Throughout the movie, the Terminator takes many gunshots and withstands punishments that would destroy a human’s frail body. The Terminator experiences a high-speed car crash and walks away nearly unharmed. His layer of flesh is damaged, but he proceeds to cut the damaged portion away and continues his mission. Near the end of the movie, the Terminator is in a fiery explosion involving a... ...us functions. In a military aspect, they could be used as guards to patrol the base, or put in the front line to lead the troops into questionable situations. In the medical field, they could be used as assistants during surgery and childbirth and once the technology is perfected, they could perform these procedures on their own. They could also be used in biohazardous material recovery and decontamination. Finally, if enough money was available, they could be purchased as maids in a household. As you can see, even though they are portrayed as an evil force in the movies, cyborgs could be a great asset to the world once the technology is perfected. Bibliography Terminator. Dir. James Cameron Perf. Arnold Schwarzenegger,Michael Biehn, Linda Hamilton. MGM, 1984. Bladerunner. Dir. Ridley Scott. Harrison Ford, Rutger Hauer. Columbia TriStar, Warner Bros., 1982.

Guiding principle for assessment of student learning Essay

Evaluation should bebased on clearly stated objectives. An objective is defined as the statement of the expected behaviors that the student should display after instruction or teaching. These objectives serve as basis for constructing and using assessment instruments to assess students’ learning. The expected behaviors are somehow related to either of the following, but not limited to: 1) knowledge; 2) reasoning; 3) skills; 4) products; or, 5) affects. Knowledge – refers to the cognitive activities which include memorizing, recalling of facts, understanding of concepts and meanings, analysing the relationships of related facts, synthesizing or related facts and principles, and evaluating the outcomes of the relationships of concepts and principles. Reasoning – is a behavior which refers to the cognitive activities which require the use of presentation, justification, and rationalization of the existence of facts, concepts, theories, and principles. Skills – defined as the ability to use once knowledge effectively and readily in the execution of performance of a specific task. This requires three elements in the skill: 1) sensing; 2) precision (accuracy); and, 3) timing. Products – refers to psychomotor activities that show the end result of using the abilities to manipulate, apply, and operate facts, concepts and principles in real-life situations. Affects – refers to the activities that show values, morals, ethics, ideals, and standards in life. B. The Principle of Appropriateness of Assessment Evaluation procedures and techniques should be selected in terms of the clearly stated objectives (Gronlund, 1981).There are various formats of assessment instruments from which the teacher can choose for classroom use. The suggested general formats are the following: Objective test – are those that require one and only one correct answer and no possible answers. Reganit, et al.(2004) posits that an objective test is made up of items for which correct responses maybe set up in advance. There are different types of objective test item formats, these are: 1) supply type; 2) matching type; 3) alternate-response type; 4) labelling; 5) enumeration; and, 6) multiple choice. Subjective test – res are evaluated by giving an opinion about the issue, concepts, ideas, and the like. In an English class, a test for the assessment of students’ writing skills are often considered as subjective because it require the teacher to rate a piece of work based on his/her personal interpretation. The raters can assign the corresponding points depending upon the quality of the piece of writing output. Performance test – when the objective of the lesson requires that at the end of the lesson the student are required to perform in an activity, the appropriate test to measure the achievement of this objective is a performance test. Oral Reasoning– when the instructional objective states that â€Å" at the end of the lesson the pupils orally defend their stand on the issue,† then an oral reasoning test is appropriate. Observation – Reed and Bergmann (2001) observation is one effective means of learning†¦how students response to classroom environment. This assessment method can be done by the teacher, co-student, or even parents. Self – Reports – students may be required to write personal opinions, journals, and reflection about a learning activity.Fromthese self-made reports, the teachers evaluate whether or not these students have learn the concepts taught in the classroom.